More than 10% of the human genome is covered by candidate cis-regulatory elements (CREs) involved in gene regulation. Dysfunction of CREs has been described as contributing to several pathologies, known as “enhanceropathies.” The CRED group focuses on deciphering the three-dimensional chromatin organisation of gene loci associated with diseases investigated at the Hospital Molecular Genetics Laboratory in Brest, particularly conditions for which current diagnostic approaches remain inconclusive (e.g., cystic fibrosis, hearing loss, intellectual disabilities…). The objective of this project is to assess the contribution of cis-regulatory elements to the regulation of these loci and to establish the concept of “cis-ruption” — dysfunction of a cis-regulatory element — as a novel mechanism underlying human disease.
Erasmus + grant available depending on eligibility criteria of the sending institution
Designing and conducting molecular biology experiments. Carrying out luciferase-based reporter gene assays. Preparing and analysing samples for high-throughput sequencing. Implementing chromatin immunoprecipitation methods (ChIP, Cut&Run). Maintaining and working with mammalian cell cultures. Contributing to chromatin conformation studies (3C/4C/5C/Hi-C). Assisting in the bioinformatic analysis of genomic and epigenomic datasets. Presenting research findings within the team and contributing to scientific discussion
This internship provides an excellent opportunity to gain experience in cutting-edge functional genomics approaches and may be extended into a doctoral research project.
Funded by the European Union. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Education and Culture Executive Agency (EACEA). Neither the European Union nor EACEA can be held responsible for them.
